PCR Reagents, Taq DNA Polymerase and Master Mix, Hot Start

Engineered for controlled polymerase activity, Accuris Hot Start Taq DNA Polymerase is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification. When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard Accuris Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without a red gel loading dye. Researchers preferring a chemically modified Hot Start polymerase, should order Accuris Hot Start ChemMod (PR1000-CM series).