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Electrophoresis Gel Visualization, SmartBlue™

 

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SmartGlow Loading Dye
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SmartGlow Loading Dye
  • SmartGlow Example in use
  • SmartBlue Blue Light Transilluminator
  • SmartBlue Mini Transilluminator
  • SmartGlow Loading Dye
 
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Electrophoresis Gel Visualization, SmartBlue™

Does your lab use electrophoresis to separate DNA or RNA molecules and need DNA stain to see your results?

Safer for samples, safer for you
Accuris SmartBlue Transilluminators are the perfect partner for the most common safe DNA stains. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few. The SmartBlue can also works for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images. Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures. In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.

Two position filter cover
Both the SmartBlue transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescinc DNA bands to be seen clearly. The covers are designed to fit flat for general gel viewing or to be placed at an angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely.

Optional amber viewing glasses
The SmartBlue Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place. These convenient glasses are recommended for users that are frequently excising DNA bands from gels. SmartBlue Plug glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

UI technology, bright and clear images
The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.

Reliable, durable and long lasting
An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue Transilluminators are covered by a 2 year warranty.

 
Accuris SmartBlue Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes. These new dyes, such as SmartGlow, SYBR Green, Gel Green, have an excitation wavelength of 465-470nm (visible blue light), and are safer to use than Ethidium Bromide.
  • Uniform illumination (UI Technology)
  • Optimal wavelength (465nm) for common safe stains
  • Easily removable, two position amber filter cover
  • Optional amber viewing glasses (E121-50)
  • Replaces hazardous Ethidium Bromide (EtBr)
  • Safer than ETBr, with less complicated disposal
  • More Sensitive than EtBr, allowing visualization as little as 0.1ng of DNA per band
  • Compatible with both UV and blue light illuminators (E121-3/3E)
  • Blue light is safer than UV for lab personnel and will not damage DNA
  • Two types available- Pre-stain (E121-1) and Loading dye (E121-2)

Ordering Info

Cat No ModelDescriptionViewing SurfaceSizePower Qty Price Qty UOM
E121-3 Blue Light TransilluminatorIncludes Amber filter cover (E121-3AC)17 x 12cm30.5 x 21.5 x 5cm120V / 50/60Hz In Stock 1 $665.24 EA
E121-3E Blue Light TransilluminatorIncludes Amber filter cover (E121-3AC)17 x 12cm30.5 x 21.5 x 5cm230V / 50/60Hz In Stock 1 $665.24 EA
E121-51 Mini Blue Light TransilluminatorIncludes Amber filter cover (E121-51AC)10.5 x 10.5cm17 x 12 x 5cm120V / 50/60Hz In Stock 1 $491.64 EA
E121-51E Mini Blue Light TransilluminatorIncludes Amber filter cover (E121-51AC)10.5 x 10.5cm17 x 12 x 5cm230V / 50/60Hz In Stock 1 $491.64 EA

Options

Cat No Description Qty/UOM Price Qty UOM
E121-1 SmartGlow Pre Stain (add to gel solution before pouring the gel) In Stock 1 $124.95 EA
E121-2 SmartGlow Loading Dye with Stain (add to DNA sample before pipetting into the gel) In Stock 1 $124.95 EA
E121-3AC SmartBlue Replacement Amber Cover (E121-3/3E) In Stock 1 $43.96 EA
E121-51AC SmartBlue Mini Replacement Amber Cover (E121-51/51E) In Stock 1 $43.10 EA
 

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