Empore™ 96-Well SPE Extraction Plates, Mixed Phase Cation (MPC)
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Empore™ 96-Well SPE Extraction Plates, Mixed Phase Cation (MPC)
96-Well Plates
Empore™ 96-Well Plates provide a means of high throughput sample preparation by processing 96 samples in a standard 8x12 microcolumn plate format compatible with standard 96-well plate liquid handling technologies and injection systems. Empore 96-Well Plates are available for solid phase extraction (SPE) and for filtration after protein precipitation.
Extraction Disk Plates for SPE
Empore High Performance Extraction Disk Plates are designed for solid phase extraction (SPE) of analytes from biological samples. Parallel sample processing allows 96 samples to be extracted in approximately one hour or less. Each well of the plate contains a standard density Empore particle-loaded membrane for efficient sample extraction. Elution can be accomplished in as little as 100µL with mobile phase or mobile phase compatible solutions for direct injection onto chromatographic systems. Extraction disk plates are ideal for sample preparation prior to LC/MS and other high throughput analytical instruments.
The Empore Extraction Disk Plates are available for small volume applications up to 1.2mL in the standard 96-well plates or for larger volume samples up to 2.5mL in deep well plates.
Sorbent Information
Mixed phase cation sorbent is a silica based particle that has been bonded with both a reversed phase group (octyl) and a strong cation exchange group (benzene sulfonic acid). This mixed phase chemistry, in which two primary modes of attraction are present, allows for a more efficient and selective extraction compared to traditional reversed phase techniques. Neutral and ionized analytes can be simultaneously extracted from a complex mixture and eluted as separate fractions. A mixed phase sorbent offers the following advantages
- Eluting acidic and neutral analytes separately from basic analytes
- Selecting specific chemical interactions by nature of solvent and pH optimization to yield a more efficient extraction
- Providing cleaner chromatograms by allowing contaminants either to pass through or be retained on the sorbent
Empore™ MPC-SD Standard and Deep Well Plates
Mixed phase cation sorbent is a silica based particle that has been bonded with both a reversed phase group (octyl) and a strong cation exchange group. These products are intended for use during scientific research only and are not intended for use in medical devices or in assessment and treatment of clinical patients.
Features and Benefits
- This mixed phase chemistry, in which two primary modes of attraction are present, allows for a more efficient and selective extraction compared to traditional reversed phase techniques
- Neutral and ionized analytes can be simultaneously extracted from a complex mixture and eluted as separate fractions. A mixed phase sorbent offers the following advantages
- Eluting acidic and neutral analytes separately from basic analytes.
- Selecting specific chemical interactions by nature of solvent and pH optimization to yield a more efficient extraction.
- Providing cleaner chromatograms by allowing contaminants either to pass through or be retained on the sorbent.
- Ultra-fast flow rate at 350mL/min– 2X faster than other leading brands
- Least elution volume– 1/3 of other leading brands and 1/10 of loose-packed SPE
- Highest consistency & reproducibility– 10-15% higher than Waters Oasis plates
- Least particle fines in eluates-1/10 of other leading brands to increase efficiency >25 (reducing tube clogging & system downtime
Nominal Sorbent Properties
| Sorbent |
Silica bonded with both octyl and benzene sulfonic acid groups |
| Ion Exchange |
0.07 meq/g |
| Pore Size |
60 Å |
Applications
- drugs of abuse
- antidepressants
- benzodiazepines
- antihypertensives
- antiarrhythmics
- opiates
- anticonvulsants
- antipsychotics
- stimulants
Product Usage
MPC sorbent is versatile and can be used in any of the following solid phase extraction schemes
- Use selective elution solvents to elute acidic and neutral analytes in one fraction, followed by basic analytes in a separate fraction
- Use one elution solvent to elute a single analyte (or multiple analytes) selectively
- Use as a reversed phase sorbent and adsorb cationic interferences to the sulfonic acid group to obtain cleaner extracts for analysis
The successful use of mixed phase chemistry relies on adsorbing an analyte by either reversed phase and/or cation exchange interactions. The optimal analyte typically contains a hydrophobic region and at least one amine group that can be protonated. The pH at which the sample is loaded is very important to ensure that the amine group is fully protonated (sample pH should be at least 2 units below pKa of targeted amine group). When MPC sorbent is used in the reversed phase mode for a neutral analyte, it may be substituted into an existing method that now uses C18 or C8 bonded silica. An acidic analyte can be retained using reversed phase when the pH is lowered to make the analyte neutral.
Important Notes
As a result of the dense particle loading within Empore membranes, proper use may require slightly higher vacuum or pressure than other SPE products. When used with a vacuum manifold, about 10 in Hg (254mm Hg or 34 kPa) may be needed to pass solutions through the disk cartridge.