The Eppendorf BioSpectrometer® basic makes your work easier- convenient handling, guided software procedures, operation directly on the device, pre-programmed methods, automatic evaluation of measurement data, and clear presentation of results.
The Eppendorf BioSpectrometer basic shares its simplicity, flexibility and reliability. In addition it has freely selectable wavelengths from 200?–?830nm (selectable in 1nm increments). This allows the user to define the parameters of each assay, whether it be a scan, a single-wavelength measurement or a multi-wavelength measurement. The Eppendorf BioSpectrometer basic caters to more disciplines as the wavelengths are not pre-defined and allows for non-routine assays or new assays to be easily programmed (in addition to the pre-programmed routine methods). The Eppendorf BioSpectrometer basic is a robust yet flexible ‘workhorse’.
Raw data
In general, the purity of a DNA solution is easily defined using purity ratios. It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity. For this reason, the BioSpectrometer is capable of recording a “Purity Scan” which displays the absorbance properties of a sample graphically, thus enabling fast and simple visual quality control.
Applications
- Nucleic acid quantification
- Direct protein quantification (UV 280nm)
- Microvolume measurement via µCuvette G1.0 for highly concentrated samples
- Bacteria growth measurement (OD 600)
- Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
- Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
- Free selectable wavelengths, e.g., 340nm: assays using NADPH or NAPH, 405nm: assays using para-nitrophenol, 420nm: assays using ortho-nitrophenol, 490nm: colorimetric fructose determination, 490nm: cytoxicity endpoint assay